LAMP Designer - 引物数据分析软件
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LAMP Designer能够设计用于环介导等温扩增测定的高效引物,在等温前提下扩增DNA和RNA序列,无需PCR设置。该技术依赖于自动循环和DNA聚合酶介导的链置换DNA合成。在不到一幼时的功夫内将几个DNA拷贝扩增到109.逆转录偶联LAMP可用于RNA序列的扩增。
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LAMP Designer特点:
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设计用于环介导等温扩增的引物
LAMP选取四种专门设计的引物(两个内引物和两个表引物),可鉴别指标DNA中的六个分歧区域。四种引物与指标DNA的杂交对于LAMP的效能来说是很关键的一步。因而,这四种引物的设计对于成功的测定很关键。
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预防交叉同源
LAMP Designer会自动诠释BLAST搜索了局并避开这些区域来设计与数据库拥有显着交叉同源性的引物。
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验证BLAST
能够针对数据库对引物进行BLAST搜索以验证它们的特异性。
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多沉LAMP引物组
能够查抄为序列设计的引物的多沉性。法式显示设计的引物之间不变的交叉同源性的自由能。
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导出了局
设计的引物组及其属职能够以尺度csv或excel体式导出。
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数据和数据库治理
能够创建多个项目。通过为每个尝试创建单独的项目,能够轻松治理多个尝试的数据。它守护一个用于序列信息和搜索了局的本地数据库。
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系统要求:
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| 对于Windows | 必要 | 推荐 |
| CPU | Pentium IV 1.80 GHz | ?Intel Core i3 (3rd Gen) |
| 内存 | 1GB可用RAM | 2GB或更高的可用RAM |
| 硬盘空间 | 500MB可用硬盘 | 1GB可用硬盘 |
| 屏幕分辨率 | 800×600 | 1024×768 |
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Windows支持的平台:?XP/Vista/Windows 7/Windows 8/Windows 10
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【英文介绍】
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Design Primers for Loop Mediated Isothermal Amplification
LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on auto-cycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 109 in less than an hour. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences.
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Avoid Cross Homologies
LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database.
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Verification BLAST
The primers can be BLAST searched against a database to verify their specificity.
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Multiplex LAMP Primer Set
The primers designed for a sequence can be checked for multiplexing. The free energies of the most stable cross homologies between the primers designed, is displayed by the program.
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Export Result
The designed primer sets, along with their properties, can be exported in standard csv or excel formats.
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Data & Database Management
Multiple projects can be created. Data of multiple experiments can be easily managed by creating a separate project for each experiment. It maintains a local database for sequence information and search results.
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